采用ＣＭ－ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ和ＳｅｐｈｏｓｉｌＣ８以及ＨＰＬＣ层析方法，自广西产中华眼镜蛇毒中分离纯化神经生长因子（ＮＧＦ），此ＮＧＦ经８ｄ鸡胚背根神经节体外培养证明具有促进神经纤维生长的活性。经ＨＰＬＣ及聚丙烯酰胺凝胶电泳鉴定为单一组成，经ＳＤＳＰＡＧＥ及ＨＰＬＣ测定分子量分别为２４．１ＫＤ和２４．９ＫＤ，等电聚焦电泳测得ｐＩ为８．２，氨基酸组分分析表明ＮＧＦ含酸性氨基酸较少，通过１２５｜标记ＮＧＦ测定出ＮＧＦ在生物体内主要分布于肾、肺、及周围神经 The nerve growth factor (NGF) was isolated and purified from Guangxi Zhuang Autonomous Region cobra venom using CM-Sepharose CL-6B and SephosilC8 as well as HPLC chromatographic method. The NGF was proved to have nerve fiber growth promoting activity in vitro by 8 d chick embryo dorsal root ganglion . Identified by HPLC and polyacrylamide gel electrophoresis as a single component, by SDSPAGE and HPLC molecular weights were 24.1KD and 24.9KD, isoelectric focusing electrophoresis pI was 8.2, amino acid composition analysis showed that the acidity of NGF Amino acids less, through the 125 | marker NGF determination of NGF in the body mainly distributed in the kidney, lung, and peripheral nerves