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A new phytosterone named passionsterone 1 has been isolated from the roots of Passiflora wilsonii, along with the known compound 24R-ethyl-5α-cholestane-3β,6α-diol 2. The structure of passionsterone 1 was elucidated as 24R-ethyl-5α-cholestane-3β,6α-diol-23-one by spectroscopic means.