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目的 了解我国人类免疫缺陷病毒 (HIV)感染者中是否存在不同HIV 1亚型混合感染 ,分析其传染来源。方法 应用巢式 PCR方法对 3例HIV 1感染者外周血单个核细胞中的HIV 1膜蛋白基因进行扩增 ,并对C2 V3及其邻区 36 0个核苷酸序列进行测定和分析 ,计算基因离散率和系统进化树。结果 3例感染者体内有B和C或B′和E 2种不同HIV 1亚型毒株序列 ,各亚型毒株与相应的Bcon、Ccon、Econ国际亚型毒株序列间的基因离散率分别为 5 0 7%～ 7 2 6 %、3 2 3%、5 38%～ 5 99% ;同一个体内不同亚型毒株序列间的基因离散率分别为 2 3 5 2 %、2 0 6 9%、2 8 44 % ;根据gp12 0V3环顶端四肽序列特征 ,发现有卖淫和静脉吸毒史妓女体内的sz46 1具有典型的欧美B亚型GPGR序列 ,sz8 2为GPGH ,其他毒株均为泰国B(B′)和E亚型所特有的GPGQ序列 ;进一步系统树分析显示 ,sz1 2、sz8 1、sz46 1与Bcon丛集一起 ,sz1 1和sz8 2、sz46 2分别与Econ、Ccon丛集。结论 同一个体可以同时感染不同亚型HIV 1毒株 ,多种高危行为可能是导致混合感染的主要原因
Objective To find out whether there is a mixed infection of different HIV subtypes in human immunodeficiency virus (HIV) in China and analyze the origin of the infection. Methods The HIV 1 membrane protein gene in peripheral blood mononuclear cells of 3 HIV-1 infected patients was amplified by nested-PCR. The 36 0 nucleotide sequences of C2 V3 and its adjacent regions were determined and analyzed. Gene Dispersion and Phylogenetic Tree. Results The sequences of two different HIV subtypes B and C or B ’and E in three infected patients were obtained. The genetic divergence rates among the subtypes and the corresponding Bcon, Ccon and Econ isolates Respectively, ranging from 57.7% to 72.6%, 32.3% and 53.8% to 59.9%, respectively. The gene dispersal rates among the different subtypes of the same in vivo were respectively 23.52% and 26.0% 9% and 28.4% respectively. According to the characteristics of the loop peptide at the top of gp12 0V3, sz46 1 was found to have typical GPGR sequences of European and American subtype B, sz8 2 was GPGH, and all the other strains were Further phylogenetic tree analysis showed that sz1 2, sz8 1, sz46 1 together with Bcon cluster, sz1 1 and sz8 2, sz46 2 were clustered with Econ and Ccon, respectively, in Thai B (B ’) and E subtypes. Conclusion The same individual can infect different subtypes of HIV-1 strains at the same time. A variety of high-risk behaviors may be the main cause of mixed infection