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为了研究BRD7基因对鼻咽癌细胞CNE1的影响,通过脂质体转染方法,将BRD7基因导入NPC细胞株CNE1细胞中.通过细胞生长曲线发现该基因能够抑制CNE1细胞的生长.为了探讨可能的作用机制,进而采用蛋白质组技术研究该基因对鼻咽癌蛋白质表达谱的影响,从而研究该基因在CNE1中的地位和作用.通过对过表达BRD7基因后鼻咽癌细胞系CNE1的蛋白质表达谱改变的研究,鉴定出19个差异表达蛋白,这些蛋白质包括:BCCIP(BRCA2andCDKN1A(p21(Waf1/Cip1)),FHL2(fourandahalfLIMdomains2),Chloridechannelregulatoryprotein;Hin-1(high-in-normal-1),WISP-1(connectivetissuegrowthfactorrelatedprotein),SREC-4(scav-engerreceptorexpressedbyendothelialcells-2),folatereceptor.这些差异蛋白涉及到基因表达调控、细胞黏附等众多的事件.从另一个侧面研究了BRD7基因与鼻咽癌的关系,扩展了BRD7基因的研究范围,并进一步充实了该基因做为鼻咽癌候选抑瘤基因的证据.
In order to study the effect of BRD7 gene on CNE1 cells in nasopharyngeal carcinoma cells, BRD7 gene was transfected into NPC cell line CNE1 by lipofection method.The growth curve of CNE1 cells was detected by cell growth curve.In order to explore the possible And then use proteomic technology to study the influence of this gene on the protein expression profile of nasopharyngeal carcinoma to investigate the role and location of this gene in CNE1.The protein expression profile of CNE1 after over-expression of BRD7 gene The altered studies identified 19 differentially expressed proteins including BCCIP (BRCA2 and CDKN1A (p21 (Waf1 / Cip1)), FHL2 (fourandahalfLIMdomains2), Chloridechannelregulatory protein; Hin- 1 (high-in-normal- 1), WISP- 1, connective tissue growth factor-related protein (SREC-4), folate receptor (SREC-4) and so on. These differential proteins are involved in numerous events such as gene expression regulation, cell adhesion, etc. The relationship between BRD7 gene and nasopharyngeal carcinoma BRD7 gene research scope, and further enrich the gene as NPC tumor suppressor gene evidence according to.