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应用四甲基偶氮唑盐（ＭＴＴ）比色法进行大肠癌细胞体外培养的化疗药敏检测，结果显示可检测到的２０例大肠癌标本中以５－Ｆｕ最为敏感（ＩＲ＝５４．７％±１４．１％），ＭＭＣ（ＩＲ＝４３．０％±１１．４％，Ｐ＜０．０５）和ＡＤＭ（ＩＲ＝４２．０％±１１．１％，Ｐ＜０．０５）次之，ＤＤＰ为不敏感（ＩＲ＝２５．９％±１２．８％，Ｐ＜０．０１），各药物组间的化疗影响药敏存在显著性差异，Ｐ＜０．００１。研究结果证实ＭＴＴ比色法是一种快速、准确地检测实体瘤化疗药材的有效方法，检测中预防细胞体外培养的微生物污染和增加瘤细胞的获得率是实验成功的关键。同时也为临床选择合适的大肠癌化疗药物提供了可靠的依据。
MTT colorimetric method was used to detect the sensitivity of colorectal cancer cell culture in vitro. The results showed that the detectable 20 cases of colorectal cancer were most sensitive to 5-Fu (IR=54.7). %±14.1%), MMC (IR=43.0%±11.4%, P<0.05) and ADM (IR=42.0%±11.1%, P<0.05) The DDP was insensitive (IR=25.9%±12.8%, P<0.01). There was a significant difference in susceptibility to chemotherapy between the drug groups, P<0.001. The results of the study confirmed that MTT colorimetric assay is an effective and rapid method for the detection of solid tumor chemotherapy drugs. The prevention of microbial contamination in vitro and the increase in the acquisition rate of tumor cells in assays are the key to successful experiments. At the same time, it also provides a reliable basis for clinical selection of appropriate colorectal cancer chemotherapy drugs.