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目的:研究转人B7-H3基因鳞癌细胞疫苗体外诱导抗肿瘤免疫应答的能力。方法:采用脂质体介导法将真核表达质粒pEGFP-B7-H3导入人鳞癌细胞Tca8113,经G418筛选后获得稳定高表达克隆,以丝裂霉素C(MMC)处理后,制成肿瘤细胞疫苗,经体外与人外周血淋巴细胞共同培养后,测定淋巴细胞特异性杀伤活性及对淋巴细胞产生细胞因子的影响。结果:转染B7-H3的Tca8113细胞能够高表达B7-H3蛋白。经MMC处理后,与野生型的Tca8113细胞相比,上述瘤苗能诱导淋巴细胞产生针对Tca8113的特异性杀伤作用,能显著增强淋巴细胞分泌IFN-γ的能力。结论:转B7-H3基因人鳞癌细胞疫苗能诱导有效的抗鳞癌免疫反应。
Objective: To study the ability of transduced human B7-H3 gene squamous cell carcinoma vaccine to induce anti-tumor immune response in vitro. METHODS: The eukaryotic expression plasmid pEGFP-B7-H3 was introduced into human squamous cell carcinoma cell line Tca8113 by liposome-mediated method. After G418 selection, stable high-expression clones were obtained and treated with mitomycin C (MMC). The tumor cell vaccine, after co-cultured with human peripheral blood lymphocytes in vitro, measures the specific killing activity of lymphocytes and the effect on lymphocyte production of cytokines. RESULTS: Tca8113 cells transfected with B7-H3 were able to overexpress B7-H3 protein. After MMC treatment, compared with the wild-type Tca8113 cells, the above-mentioned tumor vaccines can induce the specific killing effect of Tca8113 on lymphocytes, and can significantly enhance the ability of lymphocytes to secrete IFN-γ. Conclusion: The B7-H3 gene human squamous cell carcinoma vaccine can induce effective anti-squamous cell carcinoma immune response.