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目的研究宫颈脱落细胞中端粒酶RNA(hTERC)和C-myc基因的表达及其临床意义。方法对249例宫颈脱落细胞标本,应用荧光原位杂交(FISH)技术进行hTERC和C-myc基因检测,同时行阴道镜下多点活检和病理检查组织学制片。细胞标本分为正常组、宫颈上皮内瘤变(CIN)1组、CIN2组、CIN3组和鳞状细胞癌(SCC)组,比较液基细胞学、hTERC和C-myc基因扩增检测诊断SCC及CIN的敏感性和特异性。结果 hTERC和C-myc基因异常扩增率在正常组、CIN1组、CIN2组、CIN3组和SCC组分别为3.1%和2.3%、39.1%和26.1%、79.4%和50%、81.8%和54.5%、100%和80.0%。以阳性细胞>5.12%为界点,hTERC和C-myc基因联合检测CIN2组、CIN3组和SCC组的灵敏度为91.7%,特异度为83.6%,Youden指数为0.753。结论 hTERC基因联合C-myc基因检测对SCC及CIN诊断准确度高于任一个基因单独检测。
Objective To study the expression of telomerase RNA (hTERC) and C-myc in cervical exfoliated cells and its clinical significance. Methods 249 specimens of cervical exfoliated cells were detected by fluorescence in situ hybridization (FISH). The hTERC and C-myc genes were detected by colposcopy and colposcopy biopsy and histological examination. Cell samples were divided into normal group, CIN group 1, CIN2 group, CIN3 group and squamous cell carcinoma (SCC) group. And CIN sensitivity and specificity. Results The rates of abnormal amplification of hTERC and C-myc gene were 3.1% and 2.3%, 39.1% and 26.1%, 79.4% and 50%, 81.8% and 54.5% respectively in CIN1, CIN2, CIN3 and SCC %, 100% and 80.0%. Positive cells> 5.12% as the cutoff point, the combination of hTERC and C-myc gene detection CIN2 group, CIN3 group and SCC group was 91.7% sensitivity, specificity was 83.6%, Youden index was 0.753. Conclusion The diagnostic accuracy of hTERC gene combined with C-myc gene in SCC and CIN is higher than that of any single gene alone.