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利用Ｈｅｒｍａｎｓ引物扩增插入序列ＩＳ９８６所得之２４５ｂｐ片断是一结核分支杆菌复合体特异性片断，采用随机引物法将地戈辛标记该片断。将纯化结核分支杆菌ＤＮＡ经聚合酶链反应（ＰＣＲ）扩增，索氏转移后与该探针杂交，检测灵敏度可达１ｆｇＤＮＡ。通过对７９例痰标本、１４例结核性胸腔积液及２６例结核性关节腔积液的ＰＣＲ和ＤＮＡ索氏杂交试验比较，认为采用２４５ｂｐ探针，将ＤＮＡ索氏转移杂交与ＰＣＲ体外扩增相结合，不仅提高了结核病基因诊断的敏感性，同时也提高了诊断的特异性。
The 245 bp fragment obtained by amplifying the insertion sequence IS986 by using Hermans primer is a specific fragment of Mycobacterium tuberculosis complex, and digoxigenin is labeled by a random primer method. The purified Mycobacterium tuberculosis DNA by polymerase chain reaction (PCR) amplification, Soxhlet transfer and the probe hybridization, detection sensitivity up to 1fgDNA. By comparing the PCR and DNA Soxhlet hybridization tests of 79 cases of sputum, 14 cases of tuberculous pleural effusion and 26 cases of tuberculous effusions, it is considered that using 245bp probe, Soxhlet transfer DNA hybridization and PCR amplification in vitro The combination not only increases the sensitivity of TB gene diagnosis, but also improves the diagnostic specificity.