β-Glucuronidase相关论文
OsPRIOa is one of the well known pathogenesis-related genes in rice,and is induced by multiple plant hormones and pathog......
A constitutive expression system for directed evolution of β-glucuronidase for improved substrate sp
A noval β-glucuronidase from Penicillium purpurogenum (PGUS) could catalyze glycosidic bond to release non-reducing......
OsPR10a is one of the well known pathogenesis-related genes in rice,and is induced by multiple plant hormones and pathog......
Immobilization of β-glucuronidase: biocatalysis of glycyrrhizin to 18β-glycyrrhetinic acid and in-si
The non-covalent immobilization of β-glucuronidase enzyme obtained from Rhizopus oryzae was carried out by entrapment i......
本文通过对荣华二采区10...
A method for transient gene expression was developed for westem white pine (WWP,Pinus monticola Dougl.ex D.Don) using re......
Enhanced production of β-glucuronidase from Penicillium purpuro(g)enum Li-3 by optimizing fermentati
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Construction of a CaHPO4-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its ap
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Isolation of a strong matrix attachment region (MAR) and identification of its function in vitro and
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
The first intron (EPI) of rice 5-enolpyruvylshikimate 3-phosphate synthase gene was isolated by PCR from one clone with ......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
目的 探讨肝癌组织中β-葡萄糖醛酸酶(β-glucuronidase,β-G)mRNA的表达与临床病理因素的关系.方法 采用RT-PCR法检测25例肝癌组......
Isolation of a strong matrix attachment region (MAR) and identification of its function in vitro and
Inclusion of MARs in transgene cassettes enhances their expression and reduces position-effect variations in the transge......
分子改造是提高β-葡萄糖醛酸苷酶(PGUS-E)在生产单葡萄糖醛酸基甘草次酸(GAMG)过程中的键选择性的有效方法,但高通量筛选方法的缺失是......
A Proximal Promoter Region of Arabidopsis DREB2C Confers Tissue-specific Expression under Heat Stres
The dehydration-responsive element-binding factor 2C (DREB2C) is a member of the CBF/DREB subfamily of proteins,which co......
筛选到一株产β-葡萄糖醛酸酶的茵株HQ-10,能够以黄芩为底物,通过发酵将黄芩中的黄芩苷转化为黄芩素.通过对发酵条件进行优化,黄芩苷转......
目的:研究神经胶质瘤患者血清和脑脊液中β-葡萄糖醛酸酶(β-G)活性与β2微球蛋白(β2-MG)含量的相关性及临床意义.方法:分别应用E......
本文报道了一种对葡萄糖苷酸酶具有特异反应的荧光底物用以快速检测水样中大肠埃希氏杆菌。测试系统中,荧光底物的用量为50μg/ml......
PNPG(4-nitrophenylβ-Dglucopyranoside的简写,对-硝基苯嘴喃葡萄糖苷)法与部颁法对28个自然染菌样品进行大肠杆菌检查结果,两法全部符合者为26个样品,占样品总数的92。8%;不符合者2个......
本文研究了以海藻酸钠为载体,采用交联-包埋方式固定β-葡萄糖醛酸苷酶的固定化工艺。分别考察海藻酸钠浓度、戊二醛体积分数、氯......
采用后包埋免疫金技术,在透射电镜下识别确定White树脂包埋的结肠上皮细胞和结肠癌细胞内β-葡萄糖醛酸酶超微结构定位,和两者之间比较。结......
测定胃癌患者胃癌与非癌组织、腹腔液及血清中β-葡萄糖醛酸酶活性,并检测了胃组织中酸性磷酸酶和乳酸脱氨酶活性。认为,此三种酶......
目的观察结肠黏膜上皮细胞内β-葡萄糖醛酸酶在超微结构水平的精确定位。方法应用低温包埋、紫外线照射、胶体金标记、免疫电镜技......
从烟草基因组中克隆到两条新的MAR片段(M14和M17),序列分析表明,它们具有90%AT-box,A-box,T-box,碱基非配对区域,拓扑异构酶Ⅱ识别......
DeterminationofβglucuronidaseinhumancolorectalcarcinomacellinesFENGShuandSONGJinDanSubjectheadingscolorectalneoplasms;βg.........
利用后偶合技术进行组织化学染色,对66例不同肿瘤β-葡萄糖苷酸酶的分布进行研究,发现在上皮性肿瘤中此酶含量丰富,间叶性肿瘤除骨软骨瘤......
应用农杆菌EHA101,携带抗卡那霉素基因(氨基葡糖磷酸转移酶)和GUS基因(葡糖苷酸酶),对苹果品种‘Greensleeves’作基因导入获得成......
本文应用比色法检测唾液β-葡萄糖醛酸苷酶(β-GCD)212例。正常人该酶含量很低,42名无1例达到阳性标准。检测胃癌74例,阳性46例(62......
研究了重组β-葡萄糖醛酸苷酶分别在含疏水性离子液体1-丁基-3-甲基咪唑六氟磷酸盐([Bmim]PF6)和亲水性离子液体1-丁基-3-甲基咪唑四......
本文报告~(60)Co-γ线体外照射大鼠肺巨噬细胞(Alveolar Macrophage简称AM)对胞浆膜和溶酶体膜的损伤效应。大鼠AM在体外经~(60)Co......
Fischer 344大鼠分别按自由采食(AC 组),限饲能量(RC 组),自由采食+AFB_1(黄曲霉毒素 B_1,AT 组)和限饲能量+AFB_1(RT 组)处理16周......
目的: 建立高表达β-葡萄糖醛酸苷酶(βG)基因的肾癌模型,并观察βG前体药葡萄糖醛酸化阿霉素(DOX-GA3)对βG转染人肾癌GRC-1细胞......
<正> 睾酮是国际奥委会规定禁用的99种兴奋剂之一,由于睾酮是内源性激素,因此不能靠定性来判断运动员是否服用了睾酮,在人体激素代......
MUG用于药品中大肠杆菌的检定是一种新技术。通过406株大肠杆菌的试验结果,96.5%的菌株β-葡萄糖醛酸苷酶阳性。以20种药品人工污染大肠杆菌,用MUG法与......
对乳酸杆菌pPG质粒表达系统理想表达条件进行了探索。以干酪乳杆菌Lactobacillus casei393为宿主菌,β-葡萄糖苷酸酶(gusA)基因作为表......
β-Glucuronidase(GUS)是从大肠杆菌K-12菌株分离出的一种酸性水解酶,具有高稳定性、检测的灵敏性与简易性及其对转基因生物体无毒性......
β-Glucuronidase(GUS)是从大肠杆菌K-12菌株分离出的一种酸性水解酶,具有高稳定性、检测的灵敏性与简易性及其对转基因生物体无毒性......
内源性β-葡萄糖醛酸苷酶是溶酶体内的酸性水解酶,主要来源于多形核白细胞,是细胞外基质主要成分大分子蛋白聚糖降解的酶原之一,直接......
目前,有105种药物被国际奥委会禁止在体育比赛中使用,其中约20种属于类固醇蛋白同化激素。它们被人体服用后,多以β-葡萄糖醛酸甙......
本文对65例胆红素钙结石患者胆道(胆汁和胆囊粘膜)进行定性和定量细菌培养,测定患者胆汁的β-葡萄糖醛酸酶(β-G)活性,并观察比较......
大肠杆菌(Escherichia coli)产生的β-葡糖苷酸酶可以分解4-甲基伞形酮酰β-D-葡糖醛酸苷(4-MUG),形成产生荧光的终产物:4-甲基伞......
为提高重组β-葡萄糖醛酸苷酶的底物特异性,通过易错PCR方法对其进行突变并构建突变体文库,利用薄层层析和高效液相色谱对突变文库进......
In mammals, myeloid progenitors infiltrate the developing central nervous system (CNS), through the immature blood-brain......
本文对82例原发性肝癌(PHC),48例肝血管瘤(HH)和正常对照者的4种血清酶活性进行了测定,经微机统计分析,求得对PHC和HH的鉴别结果;单独检测的鉴别率:血清胆碱......
用转AtHSP18.2 promoter/GUS基因的转基因拟南芥为材料,体内(in vivo)实验证明钙-钙调素调节热激基因的表达.结果表明,无论在热激......
Actinomycetoma syndrome by Actinomadura (A.) madurae is characterized by a subcutaneous chronic lesion that affects fasc......
大鼠经每日腹腔内注射50mg/kg的丙烯酰胺共10次后,出现双后肢瘫痪等亚急性中毒症状。于自染毒起第5、12、21、36和50天分别对大鼠......
A method for transient gene expression was developed for western white pine(WWP,Pinus monticola Dougl.ex D.Don)using rep......
Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobact......