【摘 要】
：
The H1N1/2009 influenza virus has the potential to cause a human pandemic,and sporadic cases of human-to-pig transmission have been reported.In this study,t
【机 构】
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StateKeyLaboratoryofAgricultureMicrobiology,HuazhongAgricultureUniversity,Wuhan430070,PRChina
【出 处】
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第九届全国微生物学青年学者学术研讨会
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The H1N1/2009 influenza virus has the potential to cause a human pandemic,and sporadic cases of human-to-pig transmission have been reported.In this study,two influenza viruses were isolated from pigs.A phylogenetic analysis showed that the A/swine/NanChang/F9/2010(H1N1) (F9/10) strain shared a high degree of homology with the pandemic H1N1/2009 virus,and A/swine/GuangDong/34/2006 (H1N1) (34/06) strains was a classical swine influenza virus.A proteomic analysis was performed to investigate possible alterations of protein expression in porcine alveolar macrophage (PAM) cells infected by the F9/10 and 34/06 viruses over different time courses.Using 2-DE in association with MALDI-TOF MS/MS,we identified 13 up-regulated and 21 down-regulated protein spots,including cytoskeleton proteins,cellular signal transduction proteins,molecular biosynthesis proteins and heat shock proteins.
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