【摘 要】
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Object To assess the negative effect of adipocytes on osteoblastic bone formation in vitro.Methods Co-culture were performed in differentiated and undifferentiated 3T3-L1 cells with primary calvarial
【机 构】
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Department of Endocrinology of Shanghai Tongji Hospital,Medicine School of Shanghai Tongji Universit
【出 处】
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中华医学会第八次全国骨质疏松和骨矿盐疾病学术会议
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Object To assess the negative effect of adipocytes on osteoblastic bone formation in vitro.Methods Co-culture were performed in differentiated and undifferentiated 3T3-L1 cells with primary calvarial osteoblasts;differentiated dipocytes with Hif1af/f osteoblasts infected with adenoviral-cre in contrast to Hif1 af/f osteoblasts infected with adenoviral-EGFP.Osteoblastic proliferation and mineralization were analyzed after cocultered as well as osteoblastic bone formation related gene and protein expression.
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