Objective This study was aimed to quantitatively reveal the morphological structure characteristics of koilocytotic cells in cervical intraepithelial neoplasias by HE staining, to find highly specific parameters applied to differentiate and classify cervical intraepithelial neoplasias, and to discuss the value of these parameters on quantitative classify cervical intraepithelial neoplasias.Methods 30 cases of cervical intraepithelial neoplasia(CIN) tissue biopsies were collected, which contained 10 cases of CIN Ⅰ ,10 cases of CIN Ⅱ and 10 cases of CIN Ⅲ.After the biopsies were stained with HE, a technique of computer image analysis, Image-Pro Plus, was applied to test the parameters respectively, including morphological parameters of area, diameter, perimeter, nuclear-vacuoles ratio, nuclear area density, area number density of nucleus and vacuoles in CIN Ⅰ , CIN Ⅱ , CIN Ⅲ, and compared the test results.Results Among these morphological parameters of CIN Ⅰ , CIN Ⅱ , CIN Ⅲ koilocytotic cells, nucleus areas are 43.66 ± 16.5 μm2 , 49.76 ± 33.78 μm2 ,45.72 ± 13.80μm2 respectively;nucleus diameters are 4.84 ±3.6μm,7.37 ±2.41 μm,7.56 ± 1.18μm respectively;nucleus perimeters are 25.44 ±4.62μm,26.41 ±8.6μm,27.43 ±3.86μm respectively;vacuoles areas are 186.67 ± 131.47μm2 217.40 ± 163.38 μ m2 、59.30 ± 18.61μm2 respectively;vacuoles diameters are 16.30 ± 4.48μm、 17.30 ± 5.54μm、 3.82 ±0.93μm respectively;vacuoles perimeters are 58.47 ± 18.19μm、62.84 ± 21.71 μm、 14.10 ± 4.65μm respectively.There are significant differences in nucleus diameters, vacuoles areas, vacuoles diameters and vacuoles perimeters among CIN Ⅰ ,CIN Ⅱ , CIN Ⅲ.Conclusions It may help in classification and quantitative diagnosis of CIN by quantitatively analysing nucleus diameters, vacuoles areas, vacuoles diameters and vacuoles perimeters of koilocytotic cell.