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  5. 基于TCR信号转导通路探讨LY294002对CD3mAb活化的T细胞及Mtb-Ag启动的gdT细胞调控作用

基于TCR信号转导通路探讨LY294002对CD3mAb活化的T细胞及Mtb-Ag启动的gdT细胞调控作用

来源 :中国免疫学会第九届全国免疫学学术大会 | 被引量 : 0次 | 上传用户：sxhainan
【摘 要】
：
目的:观察磷脂酰肌醇3激酶(PI3K)特异性抑制剂LY294002对CD3mAb活化的T细胞及Mtb-Ag激活γδT细胞的影响,探讨PI3K在CD3mAb活化的T细胞及Mtb-Ag启动的γδT细胞TCR途径信号转导中作用.方法:分离获取健康人外周血单个核细胞(PBMC),用CD3mAb(CD3单克隆抗体)、PMA+IM(佛波醇酯+离子霉素)、Mtb-Ag(结核杆菌低分子多肽抗原)刺激PBMC,采用
【作 者】
：
王克强 
【机 构】
：
泰山医学院附属医院
【出 处】
：
中国免疫学会第九届全国免疫学学术大会
【发表日期】
：
2014年8期
【关键词】
：
γδT细胞 CD69分子 信号转导 LY294002 CD3mAb PMA+IM Mtb-Ag 
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目的:观察磷脂酰肌醇3激酶(PI3K)特异性抑制剂LY294002对CD3mAb活化的T细胞及Mtb-Ag激活γδT细胞的影响,探讨PI3K在CD3mAb活化的T细胞及Mtb-Ag启动的γδT细胞TCR途径信号转导中作用.方法:分离获取健康人外周血单个核细胞(PBMC),用CD3mAb(CD3单克隆抗体)、PMA+IM(佛波醇酯+离子霉素)、Mtb-Ag(结核杆菌低分子多肽抗原)刺激PBMC,采用流式细胞术检测CD3+T细胞和γδT细胞不同时间的CD69分子的表达情况;PBMC经LY294002处理后再刺激培养,用CD3PE/CD69FITC、γδPE/CD69FITC双染后,检测CD3+T细胞和γδT细胞CD69的表达情况;计数培养扩增10天的细胞数量.
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