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目的:建立虾仁中甲醛的高效液相色谱测定法。方法:准确称取捣碎的试样约5 g(精确至0.01 g),用水提取样品中的甲醛,经过滤后,与2,4-二硝基苯肼衍生,在波长365 nm处以紫外检测器检测,外标法定量。结果:加标回收率在94.4%~99.9%,相对标准偏差均小于2%,检出限小于0.20 mg/kg。结论:本法分离度好,灵敏度高,操作简便,适用于虾仁中甲醛的测定。
Objective: To establish a method for the determination of formaldehyde in shrimp by high performance liquid chromatography. Methods: Accurately weigh about 5 g of mashed sample (accurate to 0.01 g), extract the formaldehyde from the sample with water, filter and react with 2,4-dinitrophenylhydrazine to detect UV at 365 nm Testing, external standard method. Results: The spiked recoveries ranged from 94.4% to 99.9% with relative standard deviations less than 2%. The detection limits were less than 0.20 mg / kg. Conclusion: This method has good resolution, high sensitivity and easy operation. It is suitable for the determination of formaldehyde in shrimp.