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【Objective】Sisal is one of the main economic crops in tropical and subtropical areas today. To understand the codon usage bias in sisal chloroplast genome is essential to study its genetic law, molecular phylogenetic relationships and exogenous gene expression. 【Method】Based on the 52 coding sequences, the characteristic of codon usage in chloroplast genome of Agave hybrid No.11648(H.11648) genome was studied by the software CodonW, CUSP, SPSS.【Result】The effective number of codons(Nc) ranged from 41.34 to 61.00, indicating that codon bias was weak. The third positions for all codon are preferred to pyrimidine. Significant correlation was found for Nc and GC3.·【Conclusion】The mutational pressure combined with selection influenced the pattern of synonymous codon usage across the genes in H.11648 chloroplast genomes