【摘 要】
：
A new sensitive monoclonal antibody (mAb) 1 H2 against ochratoxin A (OTA) was reported herein.This mAb belonged to the immunoglobulin G1 (k chain) isotype.In the optimized indirect competitive enzyme
【机 构】
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Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Key
【出 处】
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中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
论文部分内容阅读
A new sensitive monoclonal antibody (mAb) 1 H2 against ochratoxin A (OTA) was reported herein.This mAb belonged to the immunoglobulin G1 (k chain) isotype.In the optimized indirect competitive enzyme -linked immunosorbent assay (icELISA), 1H2 showed a 50% inhibition concentration (IC50) value of 0.058 ng/mL and a detection limit (IC10) of 0.001 ng/mL.The cross-reactivity of 1 H2 with ochratoxin B, aflatoxins, deoxynivalenol, zearalenone, T-2 toxin, or fumonisins was below 0.3 %.Based on this mAb, an immunoaffinity column (IAC)-linked icELISA was developed for OTA detection in the cereal and oil products.The working range of the assay for solid sample was 0.36-16μg/kg.The recoveries from spiked samples of IAC linked icELISA ranged from 83% to 101%.These recoveries were much higher than those of icELISA (21-78%) and in good agreement with those obtained by using the standard high-performance liquid chromatography method (87-110%).The results indicated that the mAb 1 H2 had the values for studies of OTA in the crude agricultural products.
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