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A 60-day feeding trial was carried out to investigate the effect of iron on growth, body composition and digestive enzyme activities.Diets with seven levels of iron (53.9,90.0,115.6,146.1,176.0,215.8 and 266.0mg iron kg-1 diet)were fed to Jian carp (initial weight 11.4±0.0g).Per cent weight gain(PWG),feed efficiency(FE)and protein efficiency ratio were the lowest in fish fed the basal diet(P<0.05).Body protein content was increased with the increasing iron levels (P<0.05),but moisture, lipid and ash of fish were not significantly affected by dietary iron levels(P>0.05).activities of trypsin, lipase,a-amylase,Na+,K+-ATPase, alkaline phosphatase and gamma-glutamyl transpeptidase were improved with increasing dietary iron levels.Serum iron were significantly enhanced with dietary iron levels up to 146.1mg iron kg-1 diet, and plateaued.In conclusion, iron improved digestive enzyme activities of juvenile Jian carp and the dietary iron requirement for serum iron of juvenile Jian carp(11.4 ~ 64.0g)was 147.4mg iron kg-1 diet with ferrous fumarate as the iron source.