【摘 要】
：
In HPLC analysis, basic compounds can suffer from peak tailing and poor efficiency on silica-based RP columns, due to detrimental ionic interactions with io
【机 构】
：
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, H
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
In HPLC analysis, basic compounds can suffer from peak tailing and poor efficiency on silica-based RP columns, due to detrimental ionic interactions with ionized column silanol groups which can exist on the surface in single, geminal or vicinal forms.In general, a effective strategy to fix the peak tailing caused by silanol interactions is modifying mobile phase, e.g.to low the pH of mobile phase (typically below pH 3), to increase the buffer strength of a mobile phase, and to add triethylamine (TEA), which acts as competing base, reducing the availability of stationary phase silanols and interaction of the analyte with the silanols, to the mobile phase.
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