Hyperhomocysteinemia (HHcy) is associated with liver diseases such as fatty liver and hepatic fibrosis;however,the underlying mechanism is still largely unknown.The current study aimed to explore the signaling pathway involved in HHcy-induced hepatic steatosis (HS).C57BL/6 mice were fed a highmethionine diet (HMD) for 4 and 8 weeks to establish the HHcy mouse model.