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The domesticated silkworm Bombyx mori L.has important roles in basic biological research and applied science.To explore the practical use of transgenic technology in agricultural silkworm varieties,we fused the neomycin-resistance gene(NeoR)and the green fluorescent protein gene(gfp)into the piggyBac-based transposon vector and transduced it into silkworms by sperm-mediated gene transfer(SMGT).