【摘 要】
：
目的:研究CX3CR1/p38 MAPK信号通路在糖尿病神经痛中的作用.方法:采用雄性SD大鼠(200～220 g),通过检测大鼠50％机械刺激撤足阈值的变化观察糖尿病大鼠的痛行为;Western blot和免疫组织化学观察脊髓背角CX3CR1和p-p38 MAPK的表达水平及表达部位.结果:(1)腹腔注射链脲菌素后7d和14d,CX3CR1在脊髓背角的表达明显增加,在神经元和小胶质细胞中均有表达
【机 构】
：
广州医科大学第二附属医院疼痛科,广州510260
【出 处】
：
中华医学会疼痛学分会第十一届学术年会
论文部分内容阅读
目的:研究CX3CR1/p38 MAPK信号通路在糖尿病神经痛中的作用.方法:采用雄性SD大鼠(200～220 g),通过检测大鼠50％机械刺激撤足阈值的变化观察糖尿病大鼠的痛行为;Western blot和免疫组织化学观察脊髓背角CX3CR1和p-p38 MAPK的表达水平及表达部位.结果:(1)腹腔注射链脲菌素后7d和14d,CX3CR1在脊髓背角的表达明显增加,在神经元和小胶质细胞中均有表达:(2)腹腔注射链脲菌素后14d,p-p38 MAPK在脊髓背角的表达明显增加,而且仅在小胶质细胞表达;(3)鞘内给予CX3CR1的中和抗体,糖尿病神经痛大鼠的疼痛明显减轻;4.鞘内给予CX3CL1可激活p38 MAPK,并诱导大鼠机械痛敏.
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