【摘 要】
：
Increasing evidence suggests that breast cancer development may be driven by cancer stem cells (CSCs) derived from mutated adult stem/progenitor cells, which have acquired aberrant capacity for self-r
【机 构】
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National Reference Center for Veterinarian and Comparative Oncology (CEROVEC) Istituto Zooprofilatti
【出 处】
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(BITs 3rd Annual World Cancer Congress-2010, Breast Cancer C
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Increasing evidence suggests that breast cancer development may be driven by cancer stem cells (CSCs) derived from mutated adult stem/progenitor cells, which have acquired aberrant capacity for self-renewal, long lasting survival and proliferative activity and resistance to chemotherapeutic drugs.Feline mammary carcinomas (FMC) represent an alternative model to explore the genesis of breast cancer.Surgery-derived FMC specimens were mechanically dissociated and treated with collagenase/hyaluronidase to obtain single cell cultures, immuno-magnetically deprived of fibroblasts and seeded in stem cell-permissive medium (DMEM/F 12 containing B27, bFGF, EGF, insulin and BSA).
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