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Drug delivery systems that release the therapeutically active molecule are efficient approach to cancer gene therapy.This study is designed to investigate the effects of Lefty1-secreting and Lefty2-secreting microcapsules that mimic the embryonic microenvironment on oncotherapy.The Lefty1-secreting and Lefty2-secreting cells microcapsules are constructed successfully through stable cells transfection technology and cultured in alginate microcapsules.