Antibiotic resistance in hospital-acquired infections represents a considerable burden to patients and healthcare delivered globally.β-Lactams are the most widely administered class of antibiotics and the most common mechanism of bacterial resistance is possession of a β-lactamase enzyme.Detection of β-lactamase in a wide-range of bacteria is problematic due to interferences and/or unsuitability of the method for routine analysis.ESBL producing bacteria pose a serious clinical problem because of their wide antibiotic resistance (including penicillins,most cephalosporins and monobactams) and their complex and dynamic evolution and epidemiology.