The dependence of microstructure and thermal conductivity of EB-PVD TBCs on the substrate rotation speed during deposition was investigated.The microstructure was checked by SEM and the thermal conductivity was calculated on the basis of determination of thermal diffusivity by laser flash method.The results showed the stationary deposition formed denser coating and increasing rotation speed resulted in wider columnar grains and enlarged shadowing effect which indicated much morn volume of pores.Therefore,the lowest thermal conductivity at room temperature,1.37 W/mK,was obtained for a 171μm thick specimen when the rotation speed is 20 rpm in this study.