• 不 限
  • 期刊论文
  • 硕博论文
  • 会议论文
  • 报 纸
  • 英文论文
  2. 您的位置
  3. 首页
  4. 会议论文
  5. On-line SPE-LC-UV method with site-directed on-column derivatization for automated determination of

On-line SPE-LC-UV method with site-directed on-column derivatization for automated determination of

来源 :第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 | 被引量 : 0次 | 上传用户：mddh9666
【摘 要】
：
A unique site-directed, i.e.in-pore and on-column derivatization method was developed for direct and fully automated analysis of aldehydes in human plasma by combining SPE (Solid Phase Extraction) wit
【作 者】
：
Hui Xu Qianqian Yu Rosa Morello Melita Fleischmann Karl-Siegfried Boos 
【机 构】
：
Laboratory of Bioseparation, Institute of Clinical Chemistry, Medical Center of the University of Mu
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
【发表日期】
：
2011年3期
【关键词】
：
On-line Solid Phase Extraction HPLC aldehydes human plasma restricted access mat 
下载到本地 , 更方便阅读
下载此文 赞助VIP
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
　　A unique site-directed, i.e.in-pore and on-column derivatization method was developed for direct and fully automated analysis of aldehydes in human plasma by combining SPE (Solid Phase Extraction) with HPLC-UV.A Restricted Access Material (RAM; LiChrospher ADS C4 (Merck) was selected as SPE-column packing for on-line sample clean-up and site-directed derivatization.High-molecular-weight interfering constituents of sample matrix (e.g.proteins) are eliminated based on size-exclusion chromatography, while low-molecular-weight target analytes (hexanal and heptanal) have free access to the internal pore surface.These properties allow a site-directed, i.e.in-pore derivatization of the extracted analytes.
其他文献
Simultaneous Determination of Quercetin and Kaempferol in the Seedlings of Arabidopsis thaliana by h
Arabidopsis thaliana is the model plant in the field of plant molecular biology, for its smallest genome in plant [1].Flavonoids, an important class of plant secondary metabolites, have many essential
会议
Arabidopsis thalianaquercetinkaempferolHPLC-ESI-TOF/MS
On-line coupling of boronate affinity chromatography,porous graphitic carbon chromatography and high
Nucleosides are essential building blocks of RNA and other related biomolecules.Normal metabolism of RNA generates free nucleosides, which can be reused to build new RNA.However, abnormal cell status
会议
Boronate affinity chromatographyporous graphitic carbonmass spectrometrynucle
Preparation and characterization of octadecyl titania for the liquid chromatography stationary phase
As a good semiconductor material which is high efficiency, innocuity and chemical stability, TiO2 has been extensively used in photocatalysis, lithium battery, sensor materials, dye degradation, cosme
会议
Octadecyl titania(ODT)liquid chromatography stationary phase
Identification of hydrophilic components of hairy root of S.miltiorrhiza by UHPLC-ESI-QTOF-MS
An ultra-high-pressure liquid chromatographic (UHPLC) method was developed for identification of hydrophilic components of hairy root of S.miltiorrhiza.The sample was separated with an Agilent Poroshe
会议
UHPLCQ-TOFhairy rootS.miltiorrhiza
Qualitative and quantitative analysis of eleven Sesquiterpene lactones in Helianthus tuberosus L.lea
The leaves of Jerusalem artichoke (Helianthus tuberosus L.) have been reported to possess anticancer activities.Sesquiterpene lactones (SLs) are the dominant active constituents of Jerusalem artichoke
会议
Jerusalem artichokeHelianthus tuberosus L.Sesquiterpene lactonesUPLC/Q-TOF-MS
Simultaneously Ultra-sensitive Quantification of Three β-casomorphins by Capillary Electrophoresis-I
More and more efforts are endeavored in the ultra-sensitive quantification of peptides and proteins, because many important peptides and proteins are present at ultra-low levels and only the quantity
会议
CE-ICP-MSβ-casomorphinpeptideseuropiumproteomics
Rapid identification of 12 flavonoid aglycones in raw root of Scutellaria baicalensis Georgi by Deso
Ambient sampling/ionization mass spectrometry (ambient MS), characterized by rapid detection capacity without the sample preparation or with little sample pretreatment, has been introduced1 and develo
会议
Ambient MSDCBIScutellaria baicalensis Georgiflavonoid aglycones
Metabolites Analysis of Icariin and Baohuoside I by Zebrafish with High Performance Liquid Chromatog
Metabolic studies of Traditional Chinese Medicine (TCMs) have been applied effectively in the major fields of TCM research.However, there are still limitations for metabolic study methods so far using
会议
ZebrafishIcariinBaohuoside ⅠMetabolismHPLC-ESI-MS
Development and optimization of a system for comprehensive two-dimensional liquid chromatography wit
Comprehensive two-dimensional liquid chromatography(LC × LC) is a powerful tool for the separation of complex samples.In LC × LC system, the second dimensional column should ideally be eluted at very
会议
Comprehensive two-dimensional liquid chromatographyRapid resolution liquid chro
Discovery of bioactive peptides from scorpion Buthus martensi venom based on the high orthogonal pur
Scorpion venom constitutes a vast library of neurotoxic peptides which can provide useful probes for exploring ion channels functions.It is estimated that more than 100,000 peptide toxins exist in 150
会议
ToxinPurificationOrthogonality