【摘 要】
：
A coccus-shaped,non-spore-forming actinobacterium,designated strain 4Q3S-3T,was isolated from a surface-sterilized bark of the mangrove plant Kandelia candel collected from Cotai Ecological Zones in M
【机 构】
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Research Center for Medicine & Biology, Zunyi Medical University, Zunyi 563003, P.R.China;Institute
【出 处】
：
第八届全国微生物资源学术暨国家微生物资源平台运行服务研讨会
论文部分内容阅读
A coccus-shaped,non-spore-forming actinobacterium,designated strain 4Q3S-3T,was isolated from a surface-sterilized bark of the mangrove plant Kandelia candel collected from Cotai Ecological Zones in Macao,China and tested by polyphasic approach to clarify its taxonomic position.This actinobacterium was Gram-stain-positive and aerobic.Neither substrate nor aerial mycella was formed,and no diffusible pigments were observed on the media tested.Strain 4Q3S-3T grew optimally without NaC1 at 28-30 ℃,pH 7.0-8.0.Phylogenetic analysis based on 16S rRNA gene sequence showed that strain 4Q3S-3T belonged to the genus Friedmanniella and shared the highest 16S rRNA gene sequence similarity with F.flava W6T (96.57 %).The DNA G+C content of strain 4Q3S-3T was 69.5 mol%.The cell-wall peptidoglycan contained LL-2,6-diaminopimelic acid and MK-9(H4) was the predominant menaquinone.The polar lipids comprised phosphatidylglycerol (PG),diphosphatidylglycerol (DPG),phosphatidylinositol (PI),an unidentified glycolipid (GL),two unidentified amino lipids (AL) and two unidentified phospholipids (PL).The predominant fatty acids were anteiso-C15∶0,iso-Cl6∶0 and iso-C15∶0.Based on phylogenetic,phenotypic and chemotaxonomic data,strain 4Q3S-3T represents a novel species of the genus Friedmanniella,for which the name Friedmanniella endophytica sp.nov.is proposed.The type strain is 4Q3S-3T (=DSM 100723T =CGMCC 4.7307T).
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