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<正>O-GlcNAc(O-linked N-acetylglucosamine)modification is a non-canonical form of protein glycosylation,which occurs intracellular and is dynamically regulated.Our lab has developed chemical labeling methods that allow quantitative profiling of O-GlcNAcylated proteins and the modification sites.Recently,by applying chemical profiling for probing O-GlcNAcylation of pluripotency transcription factors in mouse