【摘 要】
：
Cholesterol is essential for membrane function. High level of cholesterol is a major risk factor for atherosclerosis.Cholesterol is synthesized from acetyl-CoA through a cascade of enzymatic reactions
【机 构】
：
Shanghai Institute of Biochemistry and Cell Biology, CAS
【出 处】
：
第十五届亚太分子生物学网络组织年会
论文部分内容阅读
Cholesterol is essential for membrane function. High level of cholesterol is a major risk factor for atherosclerosis.Cholesterol is synthesized from acetyl-CoA through a cascade of enzymatic reactions.The biosynthesis of cholesterol is tightly regulated,mainly through two feedback regulatory mechanisms: sterolregulated degradation of HMG-CoA reductase (HMGCR) and maturation of sterol regulatory element-binding protein (SREBP).HMGCR catalyzes the conversion of HMG-CoA to mevalonate that is a rate-limiting step in cholesterol biosynthesis.Sterol promotes the degradation of HMGCR through the ubiquitin-proteasome pathway,and therefore slows down cholesterol biosynthesis.Gp7,an ER membrane-anchored ubiquitin ligase,has been identified as the E3 that ubiquitinates HMGCR.Gp78 also mediates the degradation of Insig-l protein.Insig-l and -2are key negative regulators of sterol-regulated proteolysis of SREBP that controls the expression of lipogenic genes. We generated liver-specific gp78 knockout ( L-gp78-"- ) mice and showed that although the degradation of HMGCR was blunted,SREBP was suppressed due to the elevation of Insig-l/-2,and therefore the lipid biosynthesis was decreased.
其他文献
Royal jelly (RJ) is a proteinaceous secretion of hypopharyngeal glands (HGs) in head of honeybee workers. A stock of honeybees, high RJ bees (RJBs), selected from Italian bees (ITBs) since 1980s’ in C
Mass spectrometry(MS)has become a crucial technique for the analysis of protein complexes.Native MS has traditionally examined protein subunit arrangements,while proteomics MS has focused on sequence
We develop a generalized,quantitative chemoproteomic platform that can be broadly applicable to the analyses of biorthogonal-chemically engineered PTMs.A key feature of this method is the use of light
qualitative analysis,spiking quality control peptides,and creating in-depth spectral libraries for each proteome used in the experiment.Robust and reproducible chromatographic separation using a 60-mi
glycoforms is compromised by misguided quest for numbers.Given the non-template encoded nature of glycosylation,it is arguable that whether one needs to delineate every single glycoform and glycomic e
Neurodegenerative diseases are the nerve disorders caused by gradual loss of neurons in patient brains.These diseases are now known to result,directly or indirectly,from aberrant protein folding and a
MCM,composed of six polypeptides of related structures,is a central component for the replicative helicase in eukaryotic DNA replication.We previously reported that the recombinant Mcm4/6/7 complex ha
Introduction: Cell-penetrating peptides (CPP) that possess ability to transport macromolecules across the cell membrane have been developed as vehicles for intracellular delivery of therapeutic macrom
LINC complexes are composed of SUN and KASH domain-containing proteins and bridge the inner and outer double membranes of the nuclear envelope.LINC complexes play critical roles in nuclear positioning
The synthesis of dTDP is unique because of a requirement for thymidylate kinase ( TMPK). All other dNDPs including dUDP are directly produced by ribonucleotide reductase (RNR). In tumor cells.TMPK int