【摘 要】
：
褐飞虱(Nilaparvata lugens)是重要的水稻害虫之一.长期依赖化学防治导致褐飞虱对不同类型杀虫剂产生了不同水平的抗性.明确田间褐飞虱对新烟碱类杀虫剂吡虫啉产生高水平抗性的代谢机理,能够为害虫抗药性综合治理提供支持.本研究基于褐飞虱基因组信息,通过比较室内抗性品系、田间抗性种群,以及吡虫啉诱导前后的敏感品系中,解毒代谢酶P450s的表达量差异,分析了与吡虫啉代谢相关的P450s基因或基
【机 构】
：
南京农业大学植物保护学院,农业部病虫监测与治理重点开放实验室,南京,210095
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褐飞虱(Nilaparvata lugens)是重要的水稻害虫之一.长期依赖化学防治导致褐飞虱对不同类型杀虫剂产生了不同水平的抗性.明确田间褐飞虱对新烟碱类杀虫剂吡虫啉产生高水平抗性的代谢机理,能够为害虫抗药性综合治理提供支持.本研究基于褐飞虱基因组信息,通过比较室内抗性品系、田间抗性种群,以及吡虫啉诱导前后的敏感品系中,解毒代谢酶P450s的表达量差异,分析了与吡虫啉代谢相关的P450s基因或基因家族.在室内抗性品系和田间抗性种群中,增效剂PBO对吡虫啉均具有显著的增效作用,表明P450s在褐飞虱对吡虫啉的代谢抗性中发挥着重要作用.荧光定量PCR、RNAi及重组蛋白对吡虫啉的代谢研究表明,CYP6AY1的mRNA表达量上升在褐飞虱对吡虫啉的抗性中发挥着重要作用.然而,褐飞虱P450基因信息不完整限制了褐飞虱对吡虫啉抗性机理的研究.最近,褐飞虱基因组测序工作的完成为该研究提供了大量信息.通过比较吡虫啉诱导前后敏感品系和田间抗性种群中,褐飞虱全部45个P450s的表达量变化,发现在吡虫啉处理后的敏感品系中,18个P450s表达量显著上调.其中,Clade 3家簇中上调的基因所占的比例最高,且上调4倍以上的3个基因(CYP6CS1、CYP6CW1和CYP6ER1)均属于该家簇.在田间抗性种群中,表达量变化最大的3个基因分别为CYP6CS1(9.8-fold)、CYP6ER1(7.7-fold)和CYP6AY1(5.1-fold).综上所述,P450s的过量表达是褐飞虱对吡虫啉产生抗性的主要生化机制,利用基因组信息,检测吡虫啉诱导后P450s的表达量变化,为研究害虫对新烟碱类杀虫剂抗性的代谢机制提供了重要的信息.
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