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Background: Abnormal DNA methylation of male germ line is proposed as a pessible mechanism compromising spermatogenesis of some men currently diagnosed with idiopathic infertility.Previous studies suggested that aberrant DNA methylation of several genes are associated with disruptive spermatogenesis;however,little information is available on the DNA methylation patterns of testis-specific genes in idiopathic male infertility.Methods To investigate the association between the DAZ gene methylation patterns and spermatogenic failure,we performed methylation patterns in 174 idiopathic infertile patients and 58 fertile controls by bisulfite-modified sequencing.Results We found that the methylation patterns of CpG island in DAZ gene promoter region are different between somatic cells and spermatic cells in control group.DAZ gene methylation pattern among different groups of spermatogenic status was the same in somatic cells,completely methylated,and in spermatic cells the result was concordant except for the group of azoospermia,completely unmethylated.Conclusions Our data indicate that the methylation patters of DAZ gene is not associated with spermatogenic failure and,hence,suggest that epigenetic modification of DAZ is unlikely to be involved in the etiology of spermatogenic failure.