不同检测方法对O型口蹄疫抗体检测结果的比较分析

来源 :中国畜牧兽医学会2014年学术年会 | 被引量 : 0次 | 上传用户:leezuo
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引言蹄疫是由口蹄疫病毒引起的以偶蹄动物为主的急性、热性、高度传染性疫病,世界动物卫生组织(OIE)将其列为A类疾病,我国将其列为一类动物疫病[1].疫苗免疫是预防该病的重要措施之一,免疫抗体水平监测作为疫苗免疫质量评估手段在我国得到广泛的使用.正向间接血凝试验(IHA)、液相阻断ELISA试验(LpB)、VP1结构蛋白抗体ELISA方法(VP1)是目前常用口蹄疫O型抗体检测方法,但在实际应用过程中仍存在一些问题.为更好地分析检测结果,科学理解和选择检测方法,本试验选择了进口阻断ELSIA方法(VDP)与IHA、LpB、VP1方法进行比对试验,对O型口蹄疫抗体不同检测方法之间的一致性和差异性进行研究分析.
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