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In this paper,co-citation biclustering analysis was proposed based on big data.Co-citation biclustering analysis was performed using the papers on nursing research covered in Web of Science as a source.Co-citation papers of highly cited and citing papers were clustered at the same time to explore the intellectual base and research front of nursing research.The result was visualized directly by gCluto.Co-citation biclustering analysis is more intuitive to guide researchers to understand the intellectual base and research front of certain subject and to concentrate limited energy to achieve a breakthrough in key areas.