Parkinsons disease (PD) is a progressive neurodegenerative disease related to age,which primarily results from the death of dopaminergic neurons in the substantia nigra.Increasing evidences have approved epigenetic factors play important roles in the aging and disorder of human being and animals.5-aza-deoxycytidine (5-aza-dC) as DNA methylation inhibitor and trichostatin A (TSA) as histone acetylation inhibitor were used to induce status alteration of DNA methylation and histone acetylation in cells.In our studies,SH-SY5Y cells (human),N27 cells (rat) and MN9D (mouse) were used as dopaminergic cell models that we treated with 5-aza-dC or TSA.Our results showed that treatment with 5-aza-dC or TSA led to decreased cell viability and apoptosis in dopaminergic cells.Combination of 5-aza-dC and typical neurotoxins in PD,i.e.MPPP+ 6-OHDA or rotenone,resulted in stronger damages in dopaminergic cells than either of them.These results suggested that epigenetics might play roles in in pathogenesis of Parkinsons disease through association with cell death of dopaminergic neurons.