【摘 要】
：
Bioethanol is a potential and renewable energy.Although Zymomonas mobilis is suitable for ethanol fermentation, it is unable to metabolize pentose;therefore, the resources used for ethanol fermentatio
【机 构】
：
Graduate School of Biotechnology and Bioengineering, Yuan Ze University,Taoyuan 320, Taiwan, R.O.C.
【出 处】
：
The 6th World Bioenergy Symposium(WBS 2012)(第六届国际生物能源会议)
论文部分内容阅读
Bioethanol is a potential and renewable energy.Although Zymomonas mobilis is suitable for ethanol fermentation, it is unable to metabolize pentose;therefore, the resources used for ethanol fermentation are limited.Escherichia coli are able to metabolize both hexose and pentose to growth under aerobic conditions.But, E.coli could not be able to produce ethanol due to it is mainly lack of direct synthesis genes (e.g.,pyruvate decarboxylase;PDC and alcohol dehydrogenase;ADH).Therefore, in this study, we have successfully cloned the ethanol synthesis genes (PDC and ADH) from Zymomonas mobilis and pentose utilization genes (XYL1, XYL2 and XKS1) from Pichia stipitis into E.coli ETOH1 for biosynthesis of bioethanol.Experimental results prove that the recombinant strain E.coli ETOH1 was able to synthesize significant concentration of bioethanol (8.2 g/L) using 5% of xylose as substrate.Furthermore, recombinant strain E.coli ETOH1 can obtain the highest bioethanol production (12 g/L) after the enrichment of high concentration of xylose.
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