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目的研究伊犁黑蜂蜂胶对口腔常见致龋细菌生物膜的影响,探讨其防龋效果及可能的防龋机制。方法通过结晶紫染色法测定黑蜂蜂胶对口腔常见致龋菌(变异链球菌、表兄链球菌、血链球菌、黏性放线菌、内氏放线菌)的最小生物膜清除浓度(MBEC);培养测试细菌24 h单菌生物膜,加入MBEC及以下的3个浓度配置成初始p H值为7.0的含药培养基,厌氧培养24 h后测p H值,并计算p H变化值以检测不同浓度黑蜂蜂胶对测试菌单菌生物膜产酸能力的影响。蒽酮法测定MBEC及以下的3个浓度的黑蜂蜂胶对变异链球菌24 h单菌生物膜产生水不溶性胞外多糖的影响。结果黑蜂蜂胶对变异链球菌、表兄链球菌、血链球菌、黏性放线菌、内氏放线菌的MBEC分别是6.25、1.56、3.13、0.78、0.78 mg·m L-1;黑蜂蜂胶可使各测试菌单菌生物膜Δp H降低,蜂胶各组与对照组相比差异均有统计学意义(P<0.05);在MBEC浓度时,蜂胶可使变异链球菌合成水不溶性胞外多糖的能力降低。结论伊犁黑蜂蜂胶具有一定的防龋效果,其可能的防龋机制是通过有效清除口腔主要致龋细菌单菌生物膜,抑制测试菌株产酸、合成水不溶性胞外多糖的能力起作用的。
Objective To study the effect of Ili black bee propolis on common oral cariogenic bacterial biofilm and to explore its anticaries effect and possible anticaries mechanism. Methods The minimum biofilm concentration (MBEC) of black bee propolis on oral cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis, Streptococcus sanguis, Actinomyces viscosus, Actinomyces) was determined by crystal violet staining ); Cultured bacteria 24 h single bacteria biofilm, added MBEC and the following three concentrations configured to the initial p H value of 7.0 drug-containing medium, after 24 h anaerobic incubation measured p H value and calculated p H changes Value to detect different concentrations of black bee propolis on the ability of single bacteria biofilm test acid production capacity. The anthrone method was used to determine the effect of MBEC and the following three concentrations of bee propolis on the water-insoluble extracellular polysaccharides produced by Streptococcus mutans 24 h single biofilm. Results The MBEC of black bee propolis to Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis, viscous actinomycetes and actinomycetes were respectively 6.25, 1.56, 3.13, 0.78 and 0.78 mg · m L -1; The bee propolis could decrease the Δp H of the single bacterial biofilm of each tested bacteria, the difference between the propolis groups and the control group was statistically significant (P <0.05); at the concentration of MBEC, propolis could make Streptococcus mutans synthesize water insoluble cells Exopolysaccharide reduced ability. Conclusion Ili black bee propolis has a certain effect of preventing caries. Its possible mechanism of anti-caries is through the effective elimination of single oral bacteria biofilm in the oral cavity, inhibiting the ability of the test strains to produce acid and synthesize water-insoluble exopolysaccharides.