Authentic bovine embryonic stem cells have not been established despite over two decades efforts.Recently, induced pluripotent stem cells (iPSCs) derived from somatic cells have been successfully generated from mouse, human, monkey, rat, pig, sheep, horse and cow.However, the pluripotency of large domestic animal iPS cells have not yet been tested by generation of chimera and germline transmission.Differently to virus vector, this study use piggybac transposon to reprogram bovine fetal fibroblast (BFF) cells into iPS like cells.The piggybac transposon and transposase system is simple、 virus-free and efficient.It just need transposase to catalyse insertion or excision events.Here we report that coexpress human Rarg(RAR-γ) and Lrh-1 (liver receptor homologue 1) with the four factors Oct4、 Sox2、 Klf4 and c-Myc can obtain bovine iPS like cells.These cells exhibited typical colony morphology, like human ES cells.They showed normal karyotypes.Bovine iPS like cells also express ES cell markers, including Oct4、 Sox2、 Dnmt3b、 E-cadherin、 Dppa4、 Stella and stage-specific embryonic antigen4(SSEA4), TRA-1-60, TRA-1-81.The cells were able to differentiate into all three germ layers both in vitro and in teratomas.The results showed that the piggybac is an efficient tool for iPS cell derivation.