【摘 要】
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Voltage-dependent anion channel (VDAC) is mainly located in the mitochondrial outer membrane and participates in many biological processes.In mammals,three VDAC subtypes (VDAC1, 2 and 3) have been ide
【机 构】
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Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing
【出 处】
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GTAUF2014 & APSMHA2014第六届长城泌尿男科转化医学论坛暨第七届亚太男性健康与抗衰老研究学会年会
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Voltage-dependent anion channel (VDAC) is mainly located in the mitochondrial outer membrane and participates in many biological processes.In mammals,three VDAC subtypes (VDAC1, 2 and 3) have been identified.Although VDAC has been extensively studied in various tissues and cells, there is little knowledge about the distribution and function of VDAC in male mammalian reproductive system.Several studies have demonstrated that VDAC exists in mammalian spermatozoa and is implicated in spermatogenesis, sperm maturation, motility and fertilization.However, there is no knowledge about the respective localization and function of three VDAC subtypes in human spermatozoa.In this study, we focused on the presence of VDAC2 in human spermatozoa and its possible role in the acrosomal integrity and acrosome reaction using specific anti-VDAC2 monoclonal antibody for the first time.The results exhibited that native VDAC2 existed in the membrane components of human spermatozoa.The co-incubation of spermatozoa with antiVDAC2 antibody did not affect the acrosomal integrity and acrosome reaction, but inhibited ionophore A23187-induced intracellular Ca increase.Our study suggested that VDAC2 was located in the acrosomal membrane or plasma membrane of human spermatozoa, and played putative roles in sperm functions through mediating Ca transmembrane transport.
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