Biological ion channels play an important role in the functioning of cell or living organism,which can open and close in response to ambient stimuli.Over the past decade,solid-state synthetic nanochannels develop quickly,and can be modified with kinds of molecules on the inner wall endowed with different functions.But the synthetic nanochannels mainly respond to single environmental stimuli,such as temperature,pH,and light [1-2].Recently,the work about more intelligent dual stimuli responsive nanochannels are reported [3-4].In the paper,we have fabricated a single glass conical nanochannel,modified with Poly-spironaphthoxazine polymer (P-SPO) via atom transfer radical polymerization (ATRP).Upon UV365-light irradiation of the P-SPO modified channel,the C-O bonds of the polymer brushes are broken,resulting in the generation of phenolic hydroxyl functionalities on the channel surface.Under basic symmetric electrolyte conditions,the exposed phenolic hydroxyl groups are ionized,exhibiting the cation-selective behaviour of the channel.Using the modified glass conical nanochannel,we can tune ionic transport through the nanochannel in response to light and pH (Fig.1).The P-SPO modified nanochannel can be recovered after Vis irradiation over 20 min (Fig.2).The study present here will benefit the design of nanofludic devices for nanoscale chemical delivery and intracellular drug delivery.