【摘 要】
：
目的 观察阿托伐他汀对体外诱导培养ApoE基因敲除小鼠骨髓间充质干细胞中沉默信息调节因子(Sirt1)和Runt相关转录因子(RunX2)的表达,探讨阿托伐他汀促成骨分化的作用机制.方法 预组加入不同浓度阿托伐他汀(10,100,1000nmol/L)干预6小时,对照组加入等体积的DMSO.另一部分随机分为四组,第一组为对照组(培养液加入等体积DMSO),第二组加入Sirtl阻断剂EX527(10
【机 构】
：
复旦大学附属华东医院骨松科 复旦大学放射医学研究所骨代谢研究室
【出 处】
：
中华医学会第八次全国骨质疏松和骨矿盐疾病学术会议
论文部分内容阅读
目的 观察阿托伐他汀对体外诱导培养ApoE基因敲除小鼠骨髓间充质干细胞中沉默信息调节因子(Sirt1)和Runt相关转录因子(RunX2)的表达,探讨阿托伐他汀促成骨分化的作用机制.方法 预组加入不同浓度阿托伐他汀(10,100,1000nmol/L)干预6小时,对照组加入等体积的DMSO.另一部分随机分为四组,第一组为对照组(培养液加入等体积DMSO),第二组加入Sirtl阻断剂EX527(10uM),第三组加入阿托伐他汀100nmol/l,第四组加入Sirt1阻断剂EX527(10uM)+阿托伐他汀100nmol/L.用RT-PCR检测细胞碱性磷酸酶ALP,骨钙素OCN,Sirt1及Runx2基因的表达.用Western blot检测细胞Sirt1和Runx2蛋白表达的变化.
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