【摘 要】
：
The present study was designed to investigate effect of first intron on ovalbumin promoter activity using immortalization of oviduct epithelial cells.The oviduct epithelial cells were cultured and the
【机 构】
：
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China;Col
【出 处】
：
第七次全国动物生物技术学术研讨会暨新疆畜牧科学院第六次学术年会
论文部分内容阅读
The present study was designed to investigate effect of first intron on ovalbumin promoter activity using immortalization of oviduct epithelial cells.The oviduct epithelial cells were cultured and then immortalized using pL-CTAgNeo virus.The pL-CTAgNeo virus maintained the structural integrity and growth of oviduct epithelial cells for 18 days whereas growth rate declined continuously with aging and cellular death after 7-10 days in controlled culture.The immortalized oviduct epithelial cells were transfected with pL-OV1345tPAGFP and pLOV2964tPAGFP virus, respectively.The DNA and mRNA abundance of OV 1345 virus was 46 and 14 times higher than OV2964 virus.The transcript effect of OV2964 virus was 3-fold higher than OV1345.The result showed that first intron in OV gene has a cis-regulation function for transcription.
其他文献
High-throughput RNA sequencing was performed for comprehendsively analyzing the transcriptome of the geese.A total of 28,803,759 bp of raw sequence data were generated, of which 28,730,361bp remained
本研究旨在探讨小鼠睾丸网、曲精细管和睾丸间质三种注射方法对转基因小鼠生产效率的影响.pEGFP-C1质粒经与脂质体混合后注入小鼠睾丸内,30d后观察睾丸形态和组织变化；检测精子和配种后仔鼠转基因阳性率.结果显示,三种不同注射方法对小鼠睾丸造成损伤由大到小依次为睾丸网注射、曲精细管注射和睾丸间质注射.荧光检测小鼠精子,睾丸网和曲精细管注射法阳性率分别为35.13±1.72％和15.13+1.45％,
[目的]本文用白痢鸡粪培养蝇蛆，探究蝇蛆抗菌肽对鸡白痢沙门氏菌感染雏鸡的治疗效果.[方法]研究选用艾维茵肉仔鸡120只：鸡白痢沙门氏菌感染雏鸡90只，未感染的健康雏鸡30只.分别收集各试验组鸡粪培养蝇蛆，粗提抗菌肽测定其浓度和抑菌活性，用白痢鸡粪培养的蝇蛆抗菌肽替换抗生素治疗白痢病鸡，测定其肠道细菌数量和治愈率.[结果]试验结果显示，白痢鸡粪培养的蝇蛆抗菌肽浓度与抑菌活性均显著高于健康组(P＜0.
为了证实牛Mx1蛋白是否具有抗FMDV活性，本研究克隆了牛Mx1基因，构建了pcDNA3.1/bMxl真核表达载体，将真核表达载体转染BHK-21细胞，获得了稳定表达牛Mx1蛋白的重组BHK-21细胞株，并用100TCID50O型FMDV感染重组BHK-21细胞，通过实时荧光定量PCR测定攻毒后12h,24h,36h和48h，FMDVVP1基因的相对拷贝数和TCID50。结果:与阴性对照组细胞相比
本研究在验证马子宫角浓缩细管精液人工输精方法的可行性.采用直肠触摸检查卵巢的方式确定母马发情和卵泡的发育状况和输精时间,直肠把握细管鲜精子宫角输精,排卵后6小时内按同样方法再追精一次.(结果)试验参配母马26匹,平均每匹输精2.1次,每次平均输入原精2.4ml,其中18匹受胎,情期受胎率为69.23％. (结论)结果表明：在能够准确的掌握母马卵巢卵泡发育前提下,马子宫角用浓缩细管精液人工输精可行,
目的：研究质粒介导喹诺酮类药物耐药基因qnrS的流行特性。方法：分离养殖场不同来源样品中的大肠杆菌菌株，利用CLSI推荐的药敏纸片法测定菌株耐药情况，通过质粒接合试验获得qnrS阳性接合子，测定qnrS对哇诺酮类药物MIC值的影响及其与其它类抗生素耐药基因的相关性，利用脉冲场凝胶电泳分析阳性菌株遗传进化关系。结果：环境源菌株qnrS的阳性率为29.2％，显著高于禽源菌株基因检出率，新进的雏鸡可迅速
本实验旨在研究马人工授精中子宫角细管输精效果及浓缩精液常温保存及运输方式，验证了传统胶管输精单次追精的结果.子宫角细管输精所得受胎率81.08％与胶管输精所得情期受胎率差异显著(0.01＜P＜0.05)；15ml离心管常温保存3h获得的情期受胎率较高(P＜0.01)；群牧马单次追精情期受胎率53.62％较多次输精结果差(P＜0.01).
本文采用第二代超高通量DNA测序技术结合生物信息学的方法，对来源于内蒙古阿尔巴斯型白绒山羊的绒毛生长期与静息期皮肤部位的样本进行了深度的转录组测序，对这两个时期所出现的差异表达基因进行了深入系统的分析，以便更加深入地了解绒山羊绒毛生长的分子机理。以不同种类成纤维细胞为核供体研究细胞类型以及受体山羊排卵数对绒山羊体细胞核移植效率的影响。结果表明不同类型与来源供体细胞其克降效率不尽相同，其中胎儿成纤维
DNA methylation is a key epigenetic modification in mammals, have essential and important roles in muscle development.Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor t