【摘 要】
：
The present study aimed at investigating the enhanced effects and genes expression in the cell cycle distribution and apoptosis ofU937 cells, a human pre-monocytic leukemia cell line lacking functiona
【机 构】
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Department of Environmental and Occupational Health National Cheng Kung University Medical College T
【出 处】
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BIT Life Sciences 1st Annual World Cancer Congess-2008(2008中
论文部分内容阅读
The present study aimed at investigating the enhanced effects and genes expression in the cell cycle distribution and apoptosis ofU937 cells, a human pre-monocytic leukemia cell line lacking functional p53 protein, after combined treatment with irradiation and As2O3.Using flow cytometry analysis, Western blot, and DNA fragmentation assay, the molecular basis for the effects of irradiation in combination with As2O3 in leukemia cells was investigated.Our results indicated that combined treatment led to activation of cdc-2, which is related to the expression of cyclin B.In addition, combined treatment increased apoptotic cell death in U937 cells, which is correlated with the duration of G2/M arrest, the increase in intracellular reactive oxygen species (ROS) generation, the decrease in B-cell leukemia/lymphoma 2 (Bclo2) and B-cell leukemia/lymphoma XL (Bcl-XL) levels, the loss of mitochondria membrane potential, and the activation of caspase-3.We found that combining radiiation and As2O3 may be an effective strategy against p53-deficient leukemia cells.
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