【摘 要】
：
Principal limitation of cell therapy is cell loss following transplantation because of the interplay between ischemia, inflammation, and apoptosis.We investigated the mechanism of preconditioning of m
【机 构】
：
University of Montreal,McGill University (Faculty of Experimental Medicine) Canada
【出 处】
：
2013百奥泰波兰重大疾病临床峰会
论文部分内容阅读
Principal limitation of cell therapy is cell loss following transplantation because of the interplay between ischemia, inflammation, and apoptosis.We investigated the mechanism of preconditioning of mesenchymal stem cells (MSC) with oxytocin (OT), which has been proposed as a novel strategy for enhancing therapeutic potential of these cells in ischemic heart.In this study, we demonstrate that rat MSC express binding sites for OT receptor (OTR) and OTR transcript and protein as detected by RT-PCR and immunofluorescence, respectively.During the treatment with OT (10-10 to 10-6M) , MSC respond with rapid calcium mobilization and upregulation of the protective phospho-Akt and phospho-ERKl/2 proteins.In OT stimulated cells, phospho-Akt accumulates intracellularly close to the mitochondrial marker COX4.Functional analyses reveal the involvement of Akt/ERK1/2 pathways in cell proliferation, migration, and protection against the cytotoxic and apoptotic effects of hypoxia and serum deprivation.In addition, OT preconditioning increases MSC glucose uptake.
其他文献
Reputed experts, international organizations and leading biomedical journals warn that the greatest risk to human health comes in the form of antibiotic-resistant bacteria.World Health Organizaton dec
The herpes simplex virus (HSV-2) can be acquired through sexual activity and has the ability to establish latent infection in host cells.Infection occurs when virions come in contact with abraded skin
Autoimmune mechanisms are involved in the development of diabetes.Intra bone marrow-bone marrow transplantation (IBM-BMT) + thymus transplantation (TT) has been shown to reduce the incidence of graft
Two types of antibodies are being developed to target soluble IgE in the blood and membrane-bound IgE (mIgE) on IgE-expressing B lymphoblast and memory B cells, for treating allergy and asthma.An ordi
Osteoarthritis (OA) has traditionally been considered a degenerative joint disease often initiated by biomechanical injury and its link to the aging process.Progress in molecular biology in the past 2
In the past decade, we have learned that tolerance in organ transplantation is linked to the development and pets istence of regulatory T cells (Treg).Therapeutic administration of Tregs has proven ef
Comparison of lifetime prevalence suggests higher rates of major depression in the inflammatory bowel disease (IBD).IBDs are associated with an autonomic imbalance and up to 35% of patients with ulcer
Recent studies showed that polymorphisms in interleukin 28B (IL28B) gene were associated with chronic hepatitis B virus (HBV) infection in Chinese.However, there are still no any reports in associatio
After a tightly regulated developmental program in the thymus, "mature" single positive (SP) thymocytes leave the thymus and enter the periphery.These newly arrived recent thymic emigrants (RTEs) are
In the period April 2008 to December 2011, Beijing SanBo brain Hospital confirmed 380 cases of FCD by surgery and pathology, which considered as the object of study.Based on the pathological findings