【摘 要】
：
目的:观察5-脂氧合酶活化蛋白(5-1ipoxygenase activating protein,FLAP)抑制剂MK886对人结肠癌细胞株SW480、Caco-2增殖和凋亡的影响.方法:噻唑蓝(MIT)比色法检测6.25、12.5
【机 构】
：
徐州医学院附属医院消化内科学教研室,江苏徐州221002
【出 处】
：
中国生理学会消化内分泌生殖代谢生理专业委员会2014年消化内分泌生殖代谢学术会议
论文部分内容阅读
目的:观察5-脂氧合酶活化蛋白(5-1ipoxygenase activating protein,FLAP)抑制剂MK886对人结肠癌细胞株SW480、Caco-2增殖和凋亡的影响.方法:噻唑蓝(MIT)比色法检测6.25、12.5、25、50、100、200 μmol/L MK886作用24、48、72 h对SW480和Caco-2细胞的抑制率;Annexin V-FITC/PI双染流式细胞术检测12.5、25、50、100 μmol/L MK886作用72 h的结肠癌细胞凋亡率;流式细胞仪检测12.5、25、50 μmol/L MK886作用72h的结肠癌细胞周期.
其他文献
Angiogenesis plays an important role in physiological bone growth and remodeling, as well as in pathological bone disorders such as delayed fracture repair, ost
目的 构建人睾丸特异性新基因hT279(GenBank登录号BC016750)的原核表达载体,纯化融合蛋白并以其为抗原免疫BALB/c小鼠制备抗人睾丸特异性hT279蛋白单克隆抗体(mAb),并进
Nano-or microscale copper oxide particles (CuO NP, CuO MP) are increasingly applied as catalysts or antimicrobial additives.This increases the risk of adver
Exposure to particulate matter (PM) like engineered nanoparticles (eNPs), chemicals or a combination of both has increased in the last century due to increa
Nanomaterials (NM) are increasingly used in various different commercial applications, which are differently regulated within the EU.EU Cosmetics Regulation
Lung surfactant (LS) is a complex lipid protein mixture that forms the interfacial lining in the alveoli of the lungs consequently reducing the surface tens
The investigation of molecular mechanisms involved in the interaction of nanoparticles with living cells is of particular interest with respect to nanomedic
红花为菊科红花属植物Carthamustinctorius L.的干燥花,作为活血化瘀的代表性药物在临床上使用了两千余年,主要用于治疗冠心病,脑血栓等多种血液循环障碍性疾病.其中红花黄色
衰老诱发的心肌易损状态与心肌内源性保护能力降低密切相关.我们前期研究证实,胰岛素激活kt-eNOS信号途径是重要的心肌内源性保护机制,该机制受损或者被削弱可能是心肌易损状
黏液覆盖于肠黏膜表面,可润滑肠道、抵抗病原菌入侵及减少肠腔蛋白酶对黏膜损伤等,是肠道防御微生物入侵的第一道屏障,在维持机体肠道正常功能方面有着重要的作用.结肠黏液的