基于ISSR的甘肃不同产区栽培当归遗传多样性研究

来源 :第一届当归国际学术研讨会 | 被引量 : 0次 | 上传用户:LQ0121
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目的:研究甘肃不同产区栽培当归的遗传多样性. 方法:应用ISSR分子标记技术对甘肃省41个居群的栽培当归样本进行分析,利用POPGENE32软件分析Neis基因多样性指数(He)等遗传信息参数,应用NTSYS软件构建亲缘关系UPGMA聚类图. 结果:6条引物共检测到54个位点,其中多态性位点46个,多态位点百分率(PPL)为85.19%.栽培当归居群间的Neis基因多样度指数(H)为0.2999,Shannons多样性信息指数(I)为0.4436,种群间基因分化系数(Gst)为0.9322,基因流(Nm)为0.0364,遗传距离变化范围0.0012~0.9394. 结论:甘肃栽培当归遗传多样性在物种水平上较高,居群内遗传多样性水平明显低于居群间;遗传变异主要存在于居群间;亲缘关系与地理分布无明显相关性.
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