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目的研究临床分离的多药耐药肠杆菌科细菌产超广谱β-内酰胺酶(ESBLs)基因型,为临床治疗产ESBLs的肠杆菌科细菌提供依据。方法收集2011年5月-2012年5月临床分离的27株肠杆菌科细菌,运用VITEK-2药敏试验复合板进行药敏试验,分析其耐药性,利用聚合酶链反应(PCR)技术扩增ESBLs基因blaCTX、blaTEM和blaSHV;测序结果与GenBank数据库进行比对分析,以确定ESBLs的主要基因亚型。结果27株肠杆菌科细菌均呈现多药耐药性,其对头孢菌素类、青霉素类和碳青霉烯类的耐药率为40.7%~100.0%;PCR扩增出18株细菌携带2种以上ESBLs基因,6株携带1种ESBLs基因,有3株细菌未检测到ESBLs基因;ESBLs基因亚型主要为blaCTX-M-3、blaTEM-1和blaSHV-12。结论医院多药耐药的肠杆菌科细菌产ESBLs基因较普遍,其主要亚型为blaCTX-M-3、blaTEM-1和blaSHV-12。
Objective To study the clinical isolates of multidrug-resistant Enterobacteriaceae producing extended-spectrum β-lactamase (ESBLs) genotypes for clinical treatment of ESBLs-producing Enterobacteriaceae bacteria provide the basis. Methods 27 strains of enterobacteriaceae isolated from May 2011 to May 2012 were collected and susceptible to antibacterial activity using VITEK-2 drug susceptibility test plates. Polymerase chain reaction (PCR) The ESBLs genes blaCTX, blaTEM and blaSHV were amplified and compared with the GenBank database to determine the major subtypes of ESBLs. Results The results showed that 27 strains of Enterobacteriaceae were multidrug-resistant and their resistance rates to cephalosporins, penicillins and carbapenems were 40.7% -100.0%. 18 strains of bacteria were amplified by PCR Six of the ESBLs-carrying strains carried one ESBLs gene. Three ESBLs-producing strains did not detect the ESBLs gene. The major subtypes of ESBLs were blaCTX-M-3, blaTEM-1 and blaSHV-12. Conclusion The multi-drug resistant strains of Enterobacteriaceae in hospitals have widespread ESBLs producing genes, and their major subtypes are blaCTX-M-3, blaTEM-1 and blaSHV-12.