【摘 要】
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The intramuscular fats (IMF) or Intramuscular triglycerides (IMTG) are situated interspersed throughout the skeletal muscles.The IMF also called marbling, which imparts meat with flavor and juiciness
【机 构】
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College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R.Ch
【出 处】
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第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
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The intramuscular fats (IMF) or Intramuscular triglycerides (IMTG) are situated interspersed throughout the skeletal muscles.The IMF also called marbling, which imparts meat with flavor and juiciness is one of the core criteria for judging carcass value.The quantity of IMF is influenced entirely by genetics, and recently, understanding the underline genetic bases of IMF has been a focus particularly in the beef industry.In the present study with the deep insights of ameliorating the beef quality by genetic means, the role of CCAAT/enhancer binding protein alpha (C/EBPα) gene has been investigated by over-expressing C/EBPα in bovine muscle stem cells (MSC) to initiate the adipogenic program.Prior to this, bovine MSC were isolated and induction of their differentiation into adipocyte, in cells which were exposed to dexamethasone IBMX and indomethacin, was tested in the presence of insulin and fetal bovine serum.Either ectopic expression of C/EBPα or treatment with dexamethasone and insulin was capable of inducing accumulation of fat droplets and expression of adipogenic inducer genes (LPL, PPARγ, C/EBPβ, C/EBPδ).The expressions of myoblast-related genes (MyoD, MyfS, and Pax7) were also measured to assess the accuracy of the differentiation process.This work has provided evidence that the C/EBPα gene is essential for cattle adipose tissue growth and development.Hence, this finding can contribute to improving beef carcass quality.
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