【摘 要】
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Fibrosis is a phenomenon of fibrous connective tissue increase in the organs.Activated hepatic stellate cells(HSCs) are the preferred targets of antihepatic fibrotic therapies.Ginseng and Platycodon g
【机 构】
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Key Laboratory for Natural Resource of ChangBai Mountain & Functional Molecules,Ministry of Educatio
论文部分内容阅读
Fibrosis is a phenomenon of fibrous connective tissue increase in the organs.Activated hepatic stellate cells(HSCs) are the preferred targets of antihepatic fibrotic therapies.Ginseng and Platycodon grandiflorum are important traditional Chinese medicine herbs.The study was to investigate the antifibrosis effects of ginseng extract and Platycodon grandiflorum extract in HSCs.Extract include Ginseng water extract and alcohol extract, Platycodon grandiflorum water extract and alcohol extract, combination of both water extract and alcohol extract.Activated hepatic stellate cells were treated with Ginseng and Platycodon grandiflorum extracts for 24h.The maximum concentration of the drug is 2000 mg · L1 , the lowest concentration of 7.8 mg· L1, with concentration gradient dilution.3(4, 5dimethylthiazol2yl)2, 5diphenyltetrazolium bromide (MTT) can evaluate the survival rate of cells.The expressions of SIRT1, NFκB and p53 were detected by western blot.The results showed that extract effectively decreased the HSC cells viability in concentration dependent manner.Among them, the effect of combination of both extract is best.Ginseng and Platycodon grandiflorum activated the protein expressions of SIRT1 in activated HSCs.The expression of NFκB and p53 were significantly decreased by Ginseng and Platycodon grandiflorum.This study demonstrated that ginseng extract and Platycodon grandiflorum extract showed obviously antifibrosis effect, and decreased expression of NFκB and p53 via SIRT1 signaling pathway.
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