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目的：本实验旨在观察清心饮对病毒性心肌炎（ＶＭＣ）小鼠的疗效，并初步探讨其细胞免疫调节机制。方法：选用 ＢＡＬＢ／ｃ小鼠经腹腔注射含１０~９ＴＣＩＤ５０柯萨奇Ｂ３病毒（ＣＶＢ３）的Ｅａｇｌｅ’ｓ ＭＥＭ液建立起ＶＭＣ小鼠模型。研究分为两部分。第一部分用药后通过对病毒感染后５ｄ、 １０ｄ小鼠血清肌酸磷酸激酶（ＣＰＫ）的分别检测和病理组织学检查，观察清心饮对ＶＭＣ小鼠的疗效；第二部分用药后通过分别测定病毒感染后１０ｄ小鼠脾脏中的Ｔ淋巴细胞亚群和ＮＫ细胞活性，以及血清中的细胞间粘附因子（ＩＣＡＭ～Ｉ），肿瘤坏死因子（ＴＮＦ－α）与白介素－Ⅱ（ＩＬ－２），初步探讨清心饮对ＶＭＣ小鼠细胞免疫调节机制。结果：１．清心饮能够剂量依赖性地降低ＶＭＣ小鼠血清ＣＰＫ（与模型组比，Ｐ＜０．０５～０．０１），并通过病理组织学发现清心饮能够明显减轻ＶＭＣ小鼠心肌的炎症反应和稳定心肌细胞的微观结构。２．清心饮能够明显降低ＶＭＣ小鼠脾脏中异常增高的Ｔ淋巴细胞亚群和ＮＫ细胞活性（与模型组比，Ｐ＜０．０５～０．０１），同时可以明显抑制ＶＭＣ小鼠血清中ＩＣＡＭ－Ⅰ、ＴＮＦ－α及ＩＬ－２的表达（与模型组比，Ｐ＜０．０５～０．０１）。结论：１.清心饮对
Objective: This experiment aims to observe the efficacy of Qingxinyin in mice with viral myocarditis (VMC) and preliminary explore its cellular immune regulation mechanism. Methods: BALB/c mice were injected intraperitoneally with Eagle’s MEM containing 10 to 9 TCID50 of Coxsackie B3 virus (CVB3) to establish a VMC mouse model. The study is divided into two parts. After the first part of the drug was administered, the serum creatine phosphokinase (CPK) levels of 5 days and 10 days after virus infection were detected and histopathological examination was performed to observe the effect of Qingxinyin on VMC mice; T lymphocyte subsets and NK cell activity in the spleen of mice 10 days after infection, and intercellular adhesion molecules (ICAM ~ I), tumor necrosis factor (TNF-α) and interleukin-II (IL-2) in serum To investigate the mechanism of Qingxinyin’s cellular immune regulation in VMC mice. Results: 1. Qingxinyin was able to dose-dependently reduce serum CPK in VMC mice (P<0.05 to 0.01 compared with the model group), and it was found by pathological histology that Qingxinyin could significantly reduce the myocardial inflammatory response and stability in VMC mice. Microscopic structure of myocardial cells. 2. Qingxinyin can significantly reduce abnormally elevated T-lymphocyte subsets and NK cell activity in the spleen of VMC mice (P<0.05 to 0.01 compared with the model group), and it can significantly inhibit ICAM- in the serum of VMC mice. I, TNF-α, and IL-2 expression (P<0.05 to 0.01 compared with the model group). Conclusion: 1. Qingxinyin