【摘 要】
:
目的:了解医院常见酵母样真菌类型的分布特点及其对常见抗真菌药物的耐药性特点,为临床真菌感染性疾病提供病原学诊断和合理使用抗真菌药物的参考依据.方法:采用常规培养分离念珠菌,用显色培养基和VlTEK 32全自动微生物分析仪鉴定念珠菌类型,并参照美国国家临床试验室标准化委员会(NCCLS)推荐的M27-A药敏试验方案的微量稀释法,应用氧化还原指示剂Alamarblue通过比色判定MIC值,分别检测分离
【机 构】
:
汕头大学医学院第二附属医院皮肤病学教研室 汕头大学医学院第二附属医院检验科
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目的:了解医院常见酵母样真菌类型的分布特点及其对常见抗真菌药物的耐药性特点,为临床真菌感染性疾病提供病原学诊断和合理使用抗真菌药物的参考依据.
方法:采用常规培养分离念珠菌,用显色培养基和VlTEK 32全自动微生物分析仪鉴定念珠菌类型,并参照美国国家临床试验室标准化委员会(NCCLS)推荐的M27-A药敏试验方案的微量稀释法,应用氧化还原指示剂Alamarblue通过比色判定MIC值,分别检测分离鉴定出的176株念珠菌属对4种抗真菌药物(氟胞嘧啶,伊曲康唑,两性霉素B,特比萘芬)的体外敏感性.
结果:在分离鉴定出的176株念珠菌中,白念珠菌株114株(64.8%),热带念珠菌23株(13.1%),克柔念珠菌15株(8.5%),光滑念珠菌11株(6.3%),季也蒙念珠菌8株(4.5%),葡萄牙念珠菌5株(2.8%),176株临床分离的念珠菌对抗真菌药物的MIC值呈正态分布,其中白念珠菌对氟胞嘧啶,伊曲康唑,两性霉素B,特比萘芬的耐药率分别为9.6%,8.7%,7.0%,4.4%;热带念珠菌分别为13.0%,0%,0%,4.3%;克柔念珠菌分别为13.3%,6.7%,0%,6.7%;光滑念珠菌分别为9.1%,9.1%,0%,0%;季也蒙念珠菌分别为12.5%,12.5%,0%,12.5%;葡萄牙念珠菌分别为0%,0%,0%,0%.
结论:目前该院临床分离念珠菌中仍以白念珠菌为主,其次是热带念珠菌及克柔念珠菌,比色微量稀释药敏试验方法有较好的可重复性和稳定性,且简单省时,目前常用的抗真菌药对念珠菌属均有良好的体外抑菌作用,均未产生明显的耐药性.
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